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tween 20  (TaKaRa)


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    Structured Review

    TaKaRa tween 20
    Tween 20, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 137 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tbs+t/Tris+Buffered+Saline+with+Tween+20+(TBS-T)+Tablets/pmc13183601-67-16-20
    Average 95 stars, based on 137 article reviews
    tween 20 - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Membrane:

    Article Title: PHLPP1 regulates PINK1-parkin signalling and life span.
    Article Snippet: Adaptability to intracellular or extracellular cues is essential for maintaining cellular homeostasis.. Metabolic signals intricately control the morphology and functions of mitochondria by regulating bioenergetics and metabolism.. Here, we describe the involvement of PHLPP1, a Ser/Thr phosphatase, in mitochondrial homeostasis.

    Article Title: Screening of factors inducing alveolar type 1 epithelial cells using human pluripotent stem cells
    Article Snippet: Samples were loaded on 8% polyacrylamide gel, electrophoresed in Tris-Glycine SDS buffer (Biorad1610732), and transferred to PVDF membrane (0.2 μm Merck) in Tris-Glycine buffer (Biorad 1610734) supplemented with 20% methanol. .. The membrane was blocked with 50% Blocking One-P (Nacalai Tesque 05999- 84) diluted with TBS-T (Takarabio T9142) for 30 min and incubated with primary antibodies (Table S2) diluted with Can Get Signal Immunoreaction Enhancer Solution 1 (TOYOBO NKB-101) at 4 °C overnight. .. On the following day, the membrane was washed with TBS-T three times and incubated with secondary antibodies diluted with Can Get Signal Immunoreaction Enhancer Solution 2 (TOYOBO NKB-301) for 1 h at room temperature and washed with TBS-T three times.

    Article Title: Screening of factors inducing alveolar type 1 epithelial cells using human pluripotent stem cells
    Article Snippet: Samples were loaded on 8% polyacrylamide gel, electrophoresed in Tris-Glycine SDS buffer (Biorad1610732), and transferred to PVDF membrane (0.2 μm Merck) in Tris-Glycine buffer (Biorad 1610734) supplemented with 20% methanol. .. The membrane was blocked with 50% Blocking One-P (Nacalai Tesque 05999- 84) diluted with TBS-T (Takarabio T9142) for 30 min and incubated with primary antibodies (Table S2) diluted with Can Get Signal Immunoreaction Enhancer Solution 1 (TOYOBO NKB-101) at 4 °C overnight. .. On the following day, the membrane was washed with TBS-T three times and incubated with secondary antibodies diluted with Can Get Signal Immunoreaction Enhancer Solution 2 (TOYOBO NKB-301) for 1 h at room temperature and washed with TBS-T three times.

    Article Title: Saffold virus exploits integrin αvβ8 and sulfated glycosaminoglycans as cooperative attachment receptors for infection.
    Article Snippet: Samples were separated by SDS–PAGE using 10–20% Extra PAGE One precast gels (13068-24, Nacalai Tesque) and transferred onto a polyvinylidene difluoride (PVDF) membrane (Trans-Blot Turbo Mini PVDF Transfer Packs; 1704156, Bio-Rad Laboratories) using the TransBlot Turbo Transfer System (1704150, Bio-Rad Laboratories). .. The membrane was blocked with 5% skim milk in TBS-T (T9142, Takara Bio) and incubated with primary and secondary antibod- ies. .. To detect of endogenous hamster integrin V and 8, rabbit anti-human integrin V polyclonal antibody (27096-1-AP, Proteintech) and rabbit anti-mouse integrin 8 (D1V7M) monoclonal an- tibody (88300, Cell Signaling Technology) were used, respectively.

    Western Blot:

    Article Title: PHLPP1 regulates PINK1-parkin signalling and life span.
    Article Snippet: Adaptability to intracellular or extracellular cues is essential for maintaining cellular homeostasis.. Metabolic signals intricately control the morphology and functions of mitochondria by regulating bioenergetics and metabolism.. Here, we describe the involvement of PHLPP1, a Ser/Thr phosphatase, in mitochondrial homeostasis.

    Article Title: Succinate mediates inflammation-induced adrenocortical dysfunction
    Article Snippet: .. DOI: https://doi.org/10.7554/eLife.83064 28 of 34 blocking with 5% skimmed milk in TBS- T (0.1% Tween- 20 [Sigma- Aldrich] in 1× Tris- buffered saline) for 1 hr at RT, membranes were incubated overnight at 4°C with anti- SDHB (1:1000; Sigma- Aldrich, HPA002868), anti- DNMT1 (1:1000; Cell Signaling, #5032), anti- Tubulin (1:3000; Sigma- Aldrich, T5186), or anti-β-Actin (1:1000; Cell Signaling, #4967), diluted in 5% BSA in TBS- T. After washing, membranes were incubated for 1 hr at RT with secondary antibodies: goat anti- rabbit IgG HRPconjugated (1:3000; Jackson ImmunoResearch) or goat anti- mouse IgG HRP- conjugated (1:3000; Jackson ImmunoResearch), diluted in 5% skimmed milk in TBS- T. The signal was detected using the Western Blot Ultra- Sensitive HRP Substrate (Takara) and imaged using the Fusion FX Imaging system (PeqLab Biotechnologie). .. Genomic DNA from 2×106 NCI- H295R cells was isolated with the Quick- DNA Miniprep Kit (Zymo Research).

    Blocking Assay:

    Article Title: Screening of factors inducing alveolar type 1 epithelial cells using human pluripotent stem cells
    Article Snippet: Samples were loaded on 8% polyacrylamide gel, electrophoresed in Tris-Glycine SDS buffer (Biorad1610732), and transferred to PVDF membrane (0.2 μm Merck) in Tris-Glycine buffer (Biorad 1610734) supplemented with 20% methanol. .. The membrane was blocked with 50% Blocking One-P (Nacalai Tesque 05999- 84) diluted with TBS-T (Takarabio T9142) for 30 min and incubated with primary antibodies (Table S2) diluted with Can Get Signal Immunoreaction Enhancer Solution 1 (TOYOBO NKB-101) at 4 °C overnight. .. On the following day, the membrane was washed with TBS-T three times and incubated with secondary antibodies diluted with Can Get Signal Immunoreaction Enhancer Solution 2 (TOYOBO NKB-301) for 1 h at room temperature and washed with TBS-T three times.

    Article Title: Screening of factors inducing alveolar type 1 epithelial cells using human pluripotent stem cells
    Article Snippet: Samples were loaded on 8% polyacrylamide gel, electrophoresed in Tris-Glycine SDS buffer (Biorad1610732), and transferred to PVDF membrane (0.2 μm Merck) in Tris-Glycine buffer (Biorad 1610734) supplemented with 20% methanol. .. The membrane was blocked with 50% Blocking One-P (Nacalai Tesque 05999- 84) diluted with TBS-T (Takarabio T9142) for 30 min and incubated with primary antibodies (Table S2) diluted with Can Get Signal Immunoreaction Enhancer Solution 1 (TOYOBO NKB-101) at 4 °C overnight. .. On the following day, the membrane was washed with TBS-T three times and incubated with secondary antibodies diluted with Can Get Signal Immunoreaction Enhancer Solution 2 (TOYOBO NKB-301) for 1 h at room temperature and washed with TBS-T three times.

    Article Title: Succinate mediates inflammation-induced adrenocortical dysfunction
    Article Snippet: .. DOI: https://doi.org/10.7554/eLife.83064 28 of 34 blocking with 5% skimmed milk in TBS- T (0.1% Tween- 20 [Sigma- Aldrich] in 1× Tris- buffered saline) for 1 hr at RT, membranes were incubated overnight at 4°C with anti- SDHB (1:1000; Sigma- Aldrich, HPA002868), anti- DNMT1 (1:1000; Cell Signaling, #5032), anti- Tubulin (1:3000; Sigma- Aldrich, T5186), or anti-β-Actin (1:1000; Cell Signaling, #4967), diluted in 5% BSA in TBS- T. After washing, membranes were incubated for 1 hr at RT with secondary antibodies: goat anti- rabbit IgG HRPconjugated (1:3000; Jackson ImmunoResearch) or goat anti- mouse IgG HRP- conjugated (1:3000; Jackson ImmunoResearch), diluted in 5% skimmed milk in TBS- T. The signal was detected using the Western Blot Ultra- Sensitive HRP Substrate (Takara) and imaged using the Fusion FX Imaging system (PeqLab Biotechnologie). .. Genomic DNA from 2×106 NCI- H295R cells was isolated with the Quick- DNA Miniprep Kit (Zymo Research).

    Incubation:

    Article Title: Screening of factors inducing alveolar type 1 epithelial cells using human pluripotent stem cells
    Article Snippet: Samples were loaded on 8% polyacrylamide gel, electrophoresed in Tris-Glycine SDS buffer (Biorad1610732), and transferred to PVDF membrane (0.2 μm Merck) in Tris-Glycine buffer (Biorad 1610734) supplemented with 20% methanol. .. The membrane was blocked with 50% Blocking One-P (Nacalai Tesque 05999- 84) diluted with TBS-T (Takarabio T9142) for 30 min and incubated with primary antibodies (Table S2) diluted with Can Get Signal Immunoreaction Enhancer Solution 1 (TOYOBO NKB-101) at 4 °C overnight. .. On the following day, the membrane was washed with TBS-T three times and incubated with secondary antibodies diluted with Can Get Signal Immunoreaction Enhancer Solution 2 (TOYOBO NKB-301) for 1 h at room temperature and washed with TBS-T three times.

    Article Title: Screening of factors inducing alveolar type 1 epithelial cells using human pluripotent stem cells
    Article Snippet: Samples were loaded on 8% polyacrylamide gel, electrophoresed in Tris-Glycine SDS buffer (Biorad1610732), and transferred to PVDF membrane (0.2 μm Merck) in Tris-Glycine buffer (Biorad 1610734) supplemented with 20% methanol. .. The membrane was blocked with 50% Blocking One-P (Nacalai Tesque 05999- 84) diluted with TBS-T (Takarabio T9142) for 30 min and incubated with primary antibodies (Table S2) diluted with Can Get Signal Immunoreaction Enhancer Solution 1 (TOYOBO NKB-101) at 4 °C overnight. .. On the following day, the membrane was washed with TBS-T three times and incubated with secondary antibodies diluted with Can Get Signal Immunoreaction Enhancer Solution 2 (TOYOBO NKB-301) for 1 h at room temperature and washed with TBS-T three times.

    Article Title: Saffold virus exploits integrin αvβ8 and sulfated glycosaminoglycans as cooperative attachment receptors for infection.
    Article Snippet: Samples were separated by SDS–PAGE using 10–20% Extra PAGE One precast gels (13068-24, Nacalai Tesque) and transferred onto a polyvinylidene difluoride (PVDF) membrane (Trans-Blot Turbo Mini PVDF Transfer Packs; 1704156, Bio-Rad Laboratories) using the TransBlot Turbo Transfer System (1704150, Bio-Rad Laboratories). .. The membrane was blocked with 5% skim milk in TBS-T (T9142, Takara Bio) and incubated with primary and secondary antibod- ies. .. To detect of endogenous hamster integrin V and 8, rabbit anti-human integrin V polyclonal antibody (27096-1-AP, Proteintech) and rabbit anti-mouse integrin 8 (D1V7M) monoclonal an- tibody (88300, Cell Signaling Technology) were used, respectively.

    Article Title: Succinate mediates inflammation-induced adrenocortical dysfunction
    Article Snippet: .. DOI: https://doi.org/10.7554/eLife.83064 28 of 34 blocking with 5% skimmed milk in TBS- T (0.1% Tween- 20 [Sigma- Aldrich] in 1× Tris- buffered saline) for 1 hr at RT, membranes were incubated overnight at 4°C with anti- SDHB (1:1000; Sigma- Aldrich, HPA002868), anti- DNMT1 (1:1000; Cell Signaling, #5032), anti- Tubulin (1:3000; Sigma- Aldrich, T5186), or anti-β-Actin (1:1000; Cell Signaling, #4967), diluted in 5% BSA in TBS- T. After washing, membranes were incubated for 1 hr at RT with secondary antibodies: goat anti- rabbit IgG HRPconjugated (1:3000; Jackson ImmunoResearch) or goat anti- mouse IgG HRP- conjugated (1:3000; Jackson ImmunoResearch), diluted in 5% skimmed milk in TBS- T. The signal was detected using the Western Blot Ultra- Sensitive HRP Substrate (Takara) and imaged using the Fusion FX Imaging system (PeqLab Biotechnologie). .. Genomic DNA from 2×106 NCI- H295R cells was isolated with the Quick- DNA Miniprep Kit (Zymo Research).

    other:

    Article Title: An AsCas12f-based compact genome-editing tool derived by deep mutational scanning and structural analysis.
    Article Snippet: To precipitate the proteins, 2 ml of pre-chilled 7.5 M ammonium acetate (Sigma-Aldrich A1542) was added and the samples were vortexed at high speed for 20 s or until a white, turbid solution was obtained.

    Article Title: Saffold virus exploits integrin αvβ8 and sulfated glycosaminoglycans as cooperative attachment receptors for infection
    Article Snippet: The cells were analyzed using a FACS Canto II (BD Biosciences) or a DxFLEX (Beckman Coulter) flow cytometer, and data were processed using WinMDI or Kaluza (Beckman Coulter) software.

    Saline:

    Article Title: Impact of arginine addition on protein concentration via ultrafiltration.
    Article Snippet: In this study, we examined the effects of arginine (L-ArgHCl) on ultrafiltration performance, a process with practical significance for not only research but also pharmaceutical applications.. Specifically, we assessed the yield and filtration rate of ultrafiltration using rabbit and goat polyclonal antibodies (neutral to basic isoelectric points) as well as model proteins, BSA (acidic) and lysozyme (basic).. When a 1 mg/mL protein solution was concentrated approximately 10-fold using a standard commercially available centrifugal ultrafiltration device, the addition of LArgHCl significantly improved yield at near-neutral buffer pH.

    Article Title: Succinate mediates inflammation-induced adrenocortical dysfunction
    Article Snippet: .. DOI: https://doi.org/10.7554/eLife.83064 28 of 34 blocking with 5% skimmed milk in TBS- T (0.1% Tween- 20 [Sigma- Aldrich] in 1× Tris- buffered saline) for 1 hr at RT, membranes were incubated overnight at 4°C with anti- SDHB (1:1000; Sigma- Aldrich, HPA002868), anti- DNMT1 (1:1000; Cell Signaling, #5032), anti- Tubulin (1:3000; Sigma- Aldrich, T5186), or anti-β-Actin (1:1000; Cell Signaling, #4967), diluted in 5% BSA in TBS- T. After washing, membranes were incubated for 1 hr at RT with secondary antibodies: goat anti- rabbit IgG HRPconjugated (1:3000; Jackson ImmunoResearch) or goat anti- mouse IgG HRP- conjugated (1:3000; Jackson ImmunoResearch), diluted in 5% skimmed milk in TBS- T. The signal was detected using the Western Blot Ultra- Sensitive HRP Substrate (Takara) and imaged using the Fusion FX Imaging system (PeqLab Biotechnologie). .. Genomic DNA from 2×106 NCI- H295R cells was isolated with the Quick- DNA Miniprep Kit (Zymo Research).

    Imaging:

    Article Title: Succinate mediates inflammation-induced adrenocortical dysfunction
    Article Snippet: .. DOI: https://doi.org/10.7554/eLife.83064 28 of 34 blocking with 5% skimmed milk in TBS- T (0.1% Tween- 20 [Sigma- Aldrich] in 1× Tris- buffered saline) for 1 hr at RT, membranes were incubated overnight at 4°C with anti- SDHB (1:1000; Sigma- Aldrich, HPA002868), anti- DNMT1 (1:1000; Cell Signaling, #5032), anti- Tubulin (1:3000; Sigma- Aldrich, T5186), or anti-β-Actin (1:1000; Cell Signaling, #4967), diluted in 5% BSA in TBS- T. After washing, membranes were incubated for 1 hr at RT with secondary antibodies: goat anti- rabbit IgG HRPconjugated (1:3000; Jackson ImmunoResearch) or goat anti- mouse IgG HRP- conjugated (1:3000; Jackson ImmunoResearch), diluted in 5% skimmed milk in TBS- T. The signal was detected using the Western Blot Ultra- Sensitive HRP Substrate (Takara) and imaged using the Fusion FX Imaging system (PeqLab Biotechnologie). .. Genomic DNA from 2×106 NCI- H295R cells was isolated with the Quick- DNA Miniprep Kit (Zymo Research).



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